I created the ultrahigh throughput assay "Tite-Seq" which titrates antigens against surface displayed antibodies. For each antigen titration, cells expressing antibodies are exposed to antigens and then antigens are marked with a fluorescent dye. Cells were then FACS sorted into sub-populations and NGS sequencing was performed on the antibody sequences. With some computational algorithms, I was able to determine binding affinites of 1000s of sequences in parallel.
At Novozymes, I successfully extended this technique to measuring thousands of enzyme catalytic activities, inhibition constants, and thermostabilities in parallel.
